Tuscola is a community rich in transportation assets due to its centralized location in the Midwestern United States and in East Central Illinois. Positioned at the intersection of Interstate 57, US Highway 36 and 45, the community has excellent road access. These three routes make Tuscola highly accessible to Interstates I-72, I-74 and I-80 to the north and to Interstates I-70, I-64 and I-24 to the south with additional routes north and south via I-55.

Tuscola also enjoys exceptional rail transportation assets for industry. The community is at the intersection of three Class I mainline railroads: CSX Transportation, Union Pacific and Canadian National (Illinois Central). These railroads provide access to all parts of the United States and cover significant territory in Mexico and Canada as well. Tuscola is a centralized point providing multiple avenues to meet rail transportation needs for industry.